2XMI
Catalytic domain of mouse 2',3'-cyclic nucleotide 3'- phosphodiesterase, complexed with citrate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-11-14 |
Detector | MARMOSAIC 225 mm CCD |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 41.620, 47.110, 107.100 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 32.862 - 1.740 |
R-factor | 0.1795 |
Rwork | 0.178 |
R-free | 0.20890 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1woj |
RMSD bond length | 0.006 |
RMSD bond angle | 0.938 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.000 | 1.790 |
High resolution limit [Å] | 1.740 | 1.740 |
Rmerge | 0.070 | 0.940 |
Number of reflections | 22280 | |
<I/σ(I)> | 17.68 | 2.08 |
Completeness [%] | 99.9 | 99.7 |
Redundancy | 6.95 | 6.09 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 3.5 | pH 3.5 |