2XMH
The X-ray structure of CTP:inositol-1-phosphate cytidylyltransferase from Archaeoglobus fulgidus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-06-29 |
Detector | ADSC QUANTUM 315r |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 154.680, 83.850, 127.690 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.140 - 2.400 |
R-factor | 0.2348 |
Rwork | 0.233 |
R-free | 0.27480 |
Structure solution method | SIRAS |
Starting model (for MR) | NONE |
RMSD bond length | 0.010 |
RMSD bond angle | 1.230 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | SHELX |
Refinement software | BUSTER (2.8.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.280 | 2.530 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.050 | 0.430 |
Number of reflections | 64618 | |
<I/σ(I)> | 13.4 | 2.3 |
Completeness [%] | 98.8 | 99.5 |
Redundancy | 3.5 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 1.2 M SODIUM CITRATE, 100 MM HEPES PH 8.2 . |