2XME
The X-ray structure of CTP:inositol-1-phosphate cytidylyltransferase from Archaeoglobus fulgidus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-11-13 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 2 1 |
| Unit cell lengths | 86.030, 127.550, 141.480 |
| Unit cell angles | 90.00, 90.56, 90.00 |
Refinement procedure
| Resolution | 27.670 - 1.890 |
| R-factor | 0.208 |
| Rwork | 0.206 |
| R-free | 0.23700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2xmh |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.100 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.8.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 142.490 | 1.990 |
| High resolution limit [Å] | 1.890 | 1.890 |
| Rmerge | 0.130 | 0.490 |
| Number of reflections | 219870 | |
| <I/σ(I)> | 10.1 | 1.5 |
| Completeness [%] | 90.3 | 83 |
| Redundancy | 4.3 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 1.8 M SODIUM MALONATE PH 6.5 . |
