2XMA
DEINOCOCCUS RADIODURANS ISDRA2 TRANSPOSASE RIGHT END DNA COMPLEX
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 95 |
Detector technology | IMAGE PLATE |
Collection date | 2008-03-17 |
Detector | RIGAKU RAXIS IV |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 76.827, 89.288, 90.104 |
Unit cell angles | 90.00, 111.23, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.300 |
R-factor | 0.2152 |
Rwork | 0.215 |
R-free | 0.23750 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2xm3 |
RMSD bond length | 0.006 |
RMSD bond angle | 2.772 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.370 |
High resolution limit [Å] | 2.300 | 2.300 |
Number of reflections | 50130 | |
<I/σ(I)> | 16.4 | 3.09 |
Completeness [%] | 99.6 | 99 |
Redundancy | 3.62 | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8 | COMLEX BUFFER: 35MM TRIS-HCL PH 8.O, 0.1 M NA-MALONATE 10MM MGCL2M 0.5 MM TCEP PRECIPITATING AGENT: 0.16 MM MGCL2, 0.1 M TRIS-HCL PH 7.9, 3.4M 1,6-HEXANEDIOL FROZEN IN LIQUID PROPANE W/O ADDITIONAL CRYOPROTECTION |