2XM7
Structural and Mechanistic Analysis of the Magnesium-Independent Aromatic Prenyltransferase CloQ from the Clorobiocin Biosynthetic Pathway
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-03-08 |
| Detector | ADSC CCD |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 135.340, 135.340, 98.640 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.850 - 2.220 |
| R-factor | 0.17572 |
| Rwork | 0.174 |
| R-free | 0.20959 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2xlq |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.499 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.5.0091) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 51.570 | 2.340 |
| High resolution limit [Å] | 2.220 | 2.220 |
| Rmerge | 0.100 | 0.620 |
| Number of reflections | 23029 | |
| <I/σ(I)> | 5.83 | 1.22 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.45 | 6.91 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | VAPOR DIFFUSION. PROTEIN (10 MG/ML) MIXED WITH AN EQUAL VOLUME OF 3 M SODIUM FORMATE, 2 MM DITHIOTHREITOL,100 MM HEPES PH (6.5). |
