2XM5
Structural and Mechanistic Analysis of the Magnesium-Independent Aromatic Prenyltransferase CloQ from the Clorobiocin Biosynthetic Pathway
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-07-11 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 135.450, 135.450, 99.340 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.830 - 1.850 |
| R-factor | 0.16224 |
| Rwork | 0.161 |
| R-free | 0.18597 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2xlq |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.447 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.5.0091) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.090 | 1.950 |
| High resolution limit [Å] | 1.650 | 1.850 |
| Rmerge | 0.090 | 0.540 |
| Number of reflections | 39516 | |
| <I/σ(I)> | 5.12 | 1.43 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 14.18 | 14.47 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | VAPOR DIFFUSION. PROTEIN (10 MG/ML) WAS MIXED WITH AN EQUAL VOLUME OF 3 M SODIUM FORMATE, 2 MM DITHIOTHREITOL, 100 MM HEPES PH 6.5 . |
