2XM4
Cytochrome c prime from Alcaligenes xylosoxidans: Ferrous R124E variant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X12 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X12 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 53.463, 53.463, 180.813 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.300 - 1.100 |
| R-factor | 0.14623 |
| Rwork | 0.145 |
| R-free | 0.16988 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.865 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.000 | 1.140 |
| High resolution limit [Å] | 1.100 | 1.100 |
| Rmerge | 0.080 | 0.400 |
| Number of reflections | 61966 | |
| <I/σ(I)> | 22.6 | 3 |
| Completeness [%] | 97.8 | 88.5 |
| Redundancy | 9.1 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.4 | AMMONIUM SULPHATE, HEPES PH7.5, pH 7.4 |
