2XLW
Cytochrome c prime from Alcaligenes xylosoxidans: Ferrous R124Q variant with bound NO
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-06-11 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 53.728, 53.728, 181.223 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.520 - 1.170 |
| R-factor | 0.167 |
| Rwork | 0.166 |
| R-free | 0.19100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1e84 |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.947 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.520 | 1.210 |
| High resolution limit [Å] | 1.170 | 1.170 |
| Rmerge | 0.060 | 0.360 |
| Number of reflections | 51845 | |
| <I/σ(I)> | 29 | 2.5 |
| Completeness [%] | 95.8 | 68.4 |
| Redundancy | 13.3 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | AMMONIUM SULPHATE, TRIS PH 7.5 |
