2XLL
The crystal structure of bilirubin oxidase from Myrothecium verrucaria
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-03-11 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 1 |
| Unit cell lengths | 52.793, 83.602, 143.148 |
| Unit cell angles | 89.98, 89.89, 89.90 |
Refinement procedure
| Resolution | 29.801 - 2.305 |
| R-factor | 0.172 |
| Rwork | 0.170 |
| R-free | 0.21630 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gsk |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.128 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.800 | 2.430 |
| High resolution limit [Å] | 2.310 | 2.310 |
| Rmerge | 0.060 | 0.100 |
| Number of reflections | 96851 | |
| <I/σ(I)> | 12.3 | 5.5 |
| Completeness [%] | 89.7 | 58.6 |
| Redundancy | 1.9 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.7 | 0.1 M CAPS, 1.23 M NAH2PO4 / NA2HPO4, 0.2 M LI2SO4, PH 8.7 |
