2XLH
REDUCED STRUCTURE OF R124A MUTANT OF CYTOCHROME C' FROM ALCALIGENES XYLOSOXIDANS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7B |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-02-23 |
| Detector | MARRESEARCH |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 53.659, 53.659, 181.155 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.470 - 1.140 |
| R-factor | 0.1321 |
| Rwork | 0.131 |
| R-free | 0.16109 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1e84 |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.939 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.000 | 1.180 |
| High resolution limit [Å] | 1.140 | 1.140 |
| Rmerge | 0.060 | 0.260 |
| Number of reflections | 55389 | |
| <I/σ(I)> | 22 | 3.5 |
| Completeness [%] | 96.2 | 77.8 |
| Redundancy | 4.1 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | AMMONIUM SULPHATE, TRIS-BUFFER PH 7.5 |
