2XLG
Structure and metal-loading of a soluble periplasm cupro-protein: Cu- CucA-open
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX10.1 |
| Synchrotron site | SRS |
| Beamline | PX10.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 87.413, 55.836, 63.034 |
| Unit cell angles | 90.00, 110.07, 90.00 |
Refinement procedure
| Resolution | 59.230 - 1.800 |
| R-factor | 0.19607 |
| Rwork | 0.194 |
| R-free | 0.22943 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2xl9 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.276 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0070) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.000 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.060 | 0.190 |
| Number of reflections | 26653 | |
| <I/σ(I)> | 14 | 4.1 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 3.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 0.1M TRIS PH 8.0, 20% PEG 8000 |
