2XLF
Structure and metal-loading of a soluble periplasm cupro-protein: apo- CucA-closed (SeMet)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 57.431, 142.473, 63.498 |
| Unit cell angles | 90.00, 95.14, 90.00 |
Refinement procedure
| Resolution | 40.660 - 2.300 |
| R-factor | 0.18674 |
| Rwork | 0.185 |
| R-free | 0.22864 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2xl9 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.290 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0070) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.000 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.090 | 0.310 |
| Number of reflections | 44448 | |
| <I/σ(I)> | 8.4 | 3.2 |
| Completeness [%] | 98.7 | 99.1 |
| Redundancy | 2.5 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 0.1M HEPES PH7.7, 18% PEG 8000, 1MM EDTA, PH 7.5 |
