2XL8
REDUCED STRUCTURE OF R124F MUTANT OF CYTOCHROME C' FROM ALCALIGENES XYLOSOXIDANS
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SRS BEAMLINE PX10.1 |
Synchrotron site | SRS |
Beamline | PX10.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-03-02 |
Detector | MARRESEARCH |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 53.681, 53.681, 181.205 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 46.470 - 1.140 |
R-factor | 0.14118 |
Rwork | 0.140 |
R-free | 0.16147 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1e84 |
RMSD bond length | 0.017 |
RMSD bond angle | 1.828 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 23.000 | 1.180 |
High resolution limit [Å] | 1.140 | 1.140 |
Rmerge | 0.056 | 0.219 |
Number of reflections | 56022 | |
<I/σ(I)> | 31 | 5 |
Completeness [%] | 97.4 | 83.9 |
Redundancy | 8.9 | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 294 | SULPHATE AMMONIUM, TRIS PH 7.5 |