2XKP
NtcA from Synechococcus elongatus: active and inactive
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM16 |
Synchrotron site | ESRF |
Beamline | BM16 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-02-26 |
Detector | ADSC QUANTUM 210r |
Spacegroup name | P 61 |
Unit cell lengths | 110.391, 110.391, 219.600 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 50.000 - 3.050 |
R-factor | 0.20828 |
Rwork | 0.206 |
R-free | 0.24740 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2xhk |
RMSD bond length | 0.009 |
RMSD bond angle | 1.222 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 87.700 | 3.210 |
High resolution limit [Å] | 3.050 | 3.050 |
Rmerge | 0.120 | 0.430 |
Number of reflections | 28882 | |
<I/σ(I)> | 5.6 | 1.8 |
Completeness [%] | 100.0 | 100 |
Redundancy | 11.2 | 11.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8.3 | NTCA PROTEIN WAS AT 4.7 MG/ML IN 50 MM SODIUM CITRATE PH 6.5, 0.5 M NACL, 5 MM MAGNESIUM CHLORIDE, 50 MM ARGININE HYDROCHLORIDE, 50 MM NA L-GLUTAMATE, 10 MM 2-OXOGLUTARATE. CRYSTALLIZATION SOLUTION: 0.2 M POTASSIUM CITRATE PH 8.3, 20 % PEG-3350. |