2XKA
Crystal structure of a GTPyS-form protofilament of Bacillus thuringiensis serovar israelensis TubZ
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-11-01 |
Detector | ADSC CCD |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 51.060, 85.150, 632.230 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 3.000 |
R-factor | 0.23459 |
Rwork | 0.232 |
R-free | 0.28438 |
Structure solution method | SAD |
Starting model (for MR) | NONE |
RMSD bond length | 0.011 |
RMSD bond angle | 1.333 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | SHELX |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 52.690 | 3.160 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.120 | 0.340 |
Number of reflections | 52941 | |
<I/σ(I)> | 7.4 | 3.6 |
Completeness [%] | 93.2 | 71 |
Redundancy | 3 | 2.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8.5 | TRISCL PH 8.5 200 MM MGCL2 30% PEG 400 WV |