2XJT
X-ray structure of the N-terminal domain of the flocculin Flo5 from Saccharomyces cerevisiae in complex with calcium and Man5(D1)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-07-25 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 46.320, 62.010, 106.360 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.330 - 1.200 |
| R-factor | 0.13086 |
| Rwork | 0.130 |
| R-free | 0.15316 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | UNPUBLISHED STRUCTURAL DATA |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.616 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.390 | 1.260 |
| High resolution limit [Å] | 1.200 | 1.200 |
| Rmerge | 0.040 | 0.330 |
| Number of reflections | 93590 | |
| <I/σ(I)> | 16.7 | 4.7 |
| Completeness [%] | 97.4 | 99.9 |
| Redundancy | 4.7 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.5M NACL, 0.1 M BISTRIS-PROPANE PH7.5, 20% PEG4000 |
