2XJK
Monomeric Human Cu,Zn Superoxide dismutase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I911-5 |
| Synchrotron site | MAX II |
| Beamline | I911-5 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-12-13 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 35.160, 49.480, 80.440 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 1.450 |
| R-factor | 0.16788 |
| Rwork | 0.166 |
| R-free | 0.19944 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1mfm |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.201 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.490 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rmerge | 0.070 | 0.660 |
| Number of reflections | 25245 | |
| <I/σ(I)> | 14 | 2.8 |
| Completeness [%] | 98.6 | 99.2 |
| Redundancy | 7.5 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 25% PEG 6000, 0.1M MES (PH 6.0), 0.01M ZNCL2 |
