2XJH
Structure and Copper-binding Properties of Methanobactins from Methylosinus trichosporium OB3b
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-08-01 |
| Detector | ADSC CCD |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 29.475, 29.475, 29.480 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 0.920 |
| R-factor | 0.1245 |
| R-free | 0.14050 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.020 |
| RMSD bond angle | 0.051 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHELXDE |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.840 | 0.970 |
| High resolution limit [Å] | 0.920 | 0.920 |
| Rmerge | 0.060 | 0.340 |
| Number of reflections | 9025 | |
| <I/σ(I)> | 25.1 | 5.5 |
| Completeness [%] | 95.7 | 78.8 |
| Redundancy | 11.3 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 4M SODIUM FORMATE, pH 7.5 |
