2XJ3
High resolution structure of the T55C mutant of CylR2.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2009-06-03 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 41 |
| Unit cell lengths | 63.450, 63.450, 41.200 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.870 - 1.230 |
| R-factor | 0.15 |
| R-free | 0.18400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1utx |
| RMSD bond length | 0.013 |
| RMSD bond angle | 0.031 |
| Data reduction software | XDS |
| Data scaling software | SADABS |
| Phasing software | PHASER |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.870 | 1.330 |
| High resolution limit [Å] | 1.230 | 1.230 |
| Rmerge | 0.040 | 0.200 |
| Number of reflections | 48194 | |
| <I/σ(I)> | 27.81 | 8.86 |
| Completeness [%] | 96.2 | 93.6 |
| Redundancy | 7.41 | 6.92 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | VAPOUR DIFFUSION, 50MM HEPES PH 7.0, 0.2M NACL |
