2XIV

Structure of Rv1477, hypothetical invasion protein of Mycobacterium tuberculosis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X12
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X12
Temperature [K]	100
Collection date	2009-06-11
Spacegroup name	P 21 21 21
Unit cell lengths	36.496, 65.542, 67.773
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.140 - 1.400
R-factor	0.15044
Rwork	0.149
R-free	0.17271
Structure solution method	SAD
Starting model (for MR)	NONE
RMSD bond length	0.011
RMSD bond angle	1.341
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	Auto-Rickshaw
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.140	1.480
High resolution limit [Å]	1.400	1.400
Rmerge	0.060	0.280
Number of reflections	31067
<I/σ(I)>	8.3	2.7
Completeness [%]	99.9	99.9
Redundancy	8.8	8.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		5.6		0.1 M TRI-SODIUM CITRATE PH=5.6 20% ISOPROPANOL 20% PEG 4000