2XI8

High resolution structure of native CylR2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	CCD
Collection date	2009-06-03
Detector	MARRESEARCH
Spacegroup name	P 41
Unit cell lengths	63.412, 63.412, 41.315
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.360 - 1.210
R-factor	0.1367
R-free	0.16660
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1utx
RMSD bond length	0.013
RMSD bond angle	0.035
Data reduction software	XDS
Data scaling software	SADABS
Phasing software	PHASER
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.360	1.310
High resolution limit [Å]	1.210	1.210
Rmerge	0.050	0.310
Number of reflections	48228
<I/σ(I)>	20.94	5.18
Completeness [%]	96.1	93.6
Redundancy	7.3	6.77

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7		VAPOUR DIFFUSION, 50MM HEPES PH7.0, 0.2M NACL.