2XHW
HCV-J4 NS5B Polymerase Trigonal Crystal Form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS |
| Synchrotron site | SLS |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-06-03 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 125.400, 125.400, 140.080 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.700 - 2.660 |
| R-factor | 0.172 |
| Rwork | 0.170 |
| R-free | 0.22100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1nb4 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.116 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((PHENIX.REFINE: 1.6_289)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.700 | 2.720 |
| High resolution limit [Å] | 2.600 | 2.660 |
| Rmerge | 0.200 | 0.730 |
| Number of reflections | 36792 | |
| <I/σ(I)> | 8.59 | 2.55 |
| Completeness [%] | 99.2 | 92.4 |
| Redundancy | 9.1 | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 2-6% PEG 3350, 0.2 M NAF, PH 7 |
