2XHS
Crystal structure of the ligand binding domain of Fushi tarazu factor 1 of Drosophila melanogaster.
Replaces: 2IZ2Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 6B |
| Synchrotron site | PAL/PLS |
| Beamline | 6B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | BRUKER |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 97.931, 97.931, 123.466 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 18.600 - 2.800 |
| R-factor | 0.219 |
| Rwork | 0.217 |
| R-free | 0.24600 |
| Structure solution method | MAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.023 |
| RMSD bond angle | 1.707 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.120 | 0.420 |
| Number of reflections | 25725 | |
| <I/σ(I)> | 19.08 | 2.5 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 15.3 | 10.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 1.3M SODIUM CITRATE TRI-BASIC, 0.1M HEPES PH 7.5 |
