2XHK
Crystal structure of transcription factor NtcA from Synechococcus elongatus bound to 2-oxoglutarate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-06-27 |
Detector | ADSC CCD |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 68.220, 70.810, 149.960 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.300 |
R-factor | 0.20566 |
Rwork | 0.204 |
R-free | 0.23769 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2xgx |
RMSD bond length | 0.015 |
RMSD bond angle | 1.563 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 75.000 | 2.420 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.050 | 0.440 |
Number of reflections | 32971 | |
<I/σ(I)> | 13.3 | 1.8 |
Completeness [%] | 99.8 | 100 |
Redundancy | 7.1 | 7.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 5.5 | NTCA PROTEIN WAS AT 4.7 MG/ML IN 50 MM SODIUM CITRATE PH 6.5, 0.5 M NACL, 5 MM MAGNESIUM CHLORIDE, 50 MM ARGININE HYDROCHLORIDE, 50 MM NA L-GLUTAMATE, 10 MM 2-OXOGLUTARATE (2OG). CRYSTALLIZATION SOLUTION: 0.1 M BIS-TRIS PH 5.5, 11 % PEG 10000, 0.15 M AMMONIUM ACETATE |