2XHF
Crystal structure of peroxiredoxin 5 from Alvinella pompejana
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X12 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X12 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-06-09 |
Detector | MARRESEARCH |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 39.540, 92.920, 49.340 |
Unit cell angles | 90.00, 109.55, 90.00 |
Refinement procedure
Resolution | 22.182 - 1.300 |
R-factor | 0.1517 |
Rwork | 0.150 |
R-free | 0.18410 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1hd2 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.270 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 22.200 | 1.500 |
High resolution limit [Å] | 1.300 | 1.300 |
Rmerge | 0.060 | 0.390 |
Number of reflections | 81682 | |
<I/σ(I)> | 13 | 3.4 |
Completeness [%] | 99.2 | 99.4 |
Redundancy | 3.9 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | HANGING DROP RESERVOIR: PEG 14000 17%(W/V), DTT 0.001M, MES PH 6.5 0.1M |