2XHC

Crystal Structure of Thermotoga maritima N-utilization Substance G (NusG)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MPG/DESY, HAMBURG BEAMLINE BW6
Synchrotron site	MPG/DESY, HAMBURG
Beamline	BW6
Temperature [K]	100
Detector technology	IMAGE PLATE
Detector	MARRESEARCH
Spacegroup name	P 43 2 2
Unit cell lengths	59.750, 59.750, 310.135
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.712 - 2.450
R-factor	0.2204
Rwork	0.217
R-free	0.27670
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2xha
RMSD bond length	0.006
RMSD bond angle	1.036
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.440
High resolution limit [Å]	2.400	2.400
Rmerge	0.070	0.570
Number of reflections	23437
<I/σ(I)>	16.6	2
Completeness [%]	99.2	99.6
Redundancy	4.9	4.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1