2XHB
Crystal structure of DNA polymerase from Thermococcus gorgonarius in complex with hypoxanthine-containing DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 93 |
Detector technology | CCD |
Detector | ADSC QUANTUM 315 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 79.450, 98.370, 116.630 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.010 - 2.720 |
R-factor | 0.23676 |
Rwork | 0.234 |
R-free | 0.29455 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2vwj |
RMSD bond length | 0.006 |
RMSD bond angle | 0.959 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.000 | 2.870 |
High resolution limit [Å] | 2.720 | 2.720 |
Rmerge | 0.100 | 0.250 |
Number of reflections | 23740 | |
<I/σ(I)> | 8.8 | 4.2 |
Completeness [%] | 95.1 | 97 |
Redundancy | 3.6 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 9 | 0.1M BICINE PH 9.0, 20% PEG6000 |