2XHA
Crystal Structure of Domain 2 of Thermotoga maritima N-utilization Substance G (NusG)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
| Synchrotron site | MPG/DESY, HAMBURG |
| Beamline | BW6 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.97985, 0.97893 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 136.350, 42.264, 88.360 |
| Unit cell angles | 90.00, 106.56, 90.00 |
Refinement procedure
| Resolution | 29.113 - 1.906 |
| R-factor | 0.1835 |
| Rwork | 0.181 |
| R-free | 0.22990 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.148 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELX |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.090 | 0.790 |
| Number of reflections | 37677 | |
| <I/σ(I)> | 13.3 | 1.8 |
| Completeness [%] | 99.9 | 72.7 |
| Redundancy | 3.8 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.8 | 0.1 M TRIS, PH 8.8, 0.2 M SODIUM ACETATE, 20% (W/V) PEG 3350 |
