2XH6
Clostridium perfringens enterotoxin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-02-21 |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 210.664, 119.296, 74.834 |
| Unit cell angles | 90.00, 110.50, 90.00 |
Refinement procedure
| Resolution | 63.393 - 2.690 |
| R-factor | 0.2131 |
| Rwork | 0.211 |
| R-free | 0.24920 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 2QUO AND 2ZS6 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.532 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 70.000 | 2.820 |
| High resolution limit [Å] | 2.700 | 2.680 |
| Rmerge | 0.090 | 0.360 |
| Number of reflections | 42488 | |
| <I/σ(I)> | 12 | 2.2 |
| Completeness [%] | 95.8 | 83.3 |
| Redundancy | 2.7 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 32-40% V/V DIOXANE IN WATER |
