2XH4
Engineering the enolase active site pocket: Crystal structure of the S39A D321A mutant of yeast enolase 1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2009-03-25 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 |
| Unit cell lengths | 62.421, 62.606, 100.948 |
| Unit cell angles | 87.95, 76.07, 76.43 |
Refinement procedure
| Resolution | 31.690 - 1.700 |
| R-factor | 0.236 |
| Rwork | 0.233 |
| R-free | 0.27900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2xh2 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.603 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.800 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.210 | 0.830 |
| Number of reflections | 146644 | |
| <I/σ(I)> | 8.64 | 2.65 |
| Completeness [%] | 92.4 | 91.1 |
| Redundancy | 2.01 | 1.93 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 18% PEG 6000, 0.2M MAGNESIUM CHLORIDE |
