2XGK

Virus like particle of L172W mutant of Minute Virus of Mice - the immunosuppressive strain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-BM-C
Synchrotron site	APS
Beamline	14-BM-C
Temperature [K]	100
Detector technology	CCD
Collection date	2008-12-10
Detector	ADSC CCD
Spacegroup name	C 1 2 1
Unit cell lengths	442.900, 411.100, 301.800
Unit cell angles	90.00, 95.88, 90.00

Refinement procedure

Resolution	35.000 - 4.200
R-factor	0.3029
Rwork	0.303
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1mvm
RMSD bond length	0.010
RMSD bond angle	1.000
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	GLRF
Refinement software	CNS (1.21)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.000	4.390
High resolution limit [Å]	4.200	4.200
Rmerge	0.100	0.260
Number of reflections	214730
<I/σ(I)>	5.52	1.86
Completeness [%]	55.3	29.4
Redundancy	1.5	1.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5		10 MM TRIS-HCL, 8 MM CACL2, PEG 8000, VIRUS CONCENTRATION 10 MG/ML, PH 7.5, VAPOR DIFFUSION, HANGING DROP.