2XGF
Structure of the bacteriophage T4 long tail fibre needle-shaped receptor-binding tip
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-2 |
Synchrotron site | ESRF |
Beamline | ID23-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-02-14 |
Detector | MARRESEARCH |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 157.340, 53.980, 112.750 |
Unit cell angles | 90.00, 100.43, 90.00 |
Refinement procedure
Resolution | 22.000 - 2.200 |
R-factor | 0.18184 |
Rwork | 0.179 |
R-free | 0.23813 |
Structure solution method | MAD |
Starting model (for MR) | NONE |
RMSD bond length | 0.014 |
RMSD bond angle | 1.394 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | autoSHARP |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 22.000 | 2.320 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.130 | 0.440 |
Number of reflections | 47653 | |
<I/σ(I)> | 5 | 1.6 |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 3.8 | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 5 | 10 MM HEPES-NAOH PH 7.5, 1 MM MANGANOUS CHLORIDE, 5 % (W/V) POLY-ETHYLENEGLYCOL 6000, 0.1 M SODIUM CITRATE PH 5. | ||
1 | 5 | 10 MM HEPES-NAOH PH 7.5, 1 MM MANGANOUS CHLORIDE, 5 % (W/V) POLY-ETHYLENEGLYCOL 6000, 0.1 M SODIUM CITRATE PH 5. |