2XGF

Structure of the bacteriophage T4 long tail fibre needle-shaped receptor-binding tip

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	CCD
Collection date	2010-02-14
Detector	MARRESEARCH
Spacegroup name	C 1 2 1
Unit cell lengths	157.340, 53.980, 112.750
Unit cell angles	90.00, 100.43, 90.00

Refinement procedure

Resolution	22.000 - 2.200
R-factor	0.18184
Rwork	0.179
R-free	0.23813
Structure solution method	MAD
Starting model (for MR)	NONE
RMSD bond length	0.014
RMSD bond angle	1.394
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	autoSHARP
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	22.000	2.320
High resolution limit [Å]	2.200	2.200
Rmerge	0.130	0.440
Number of reflections	47653
<I/σ(I)>	5	1.6
Completeness [%]	99.9	99.9
Redundancy	3.8	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		5		10 MM HEPES-NAOH PH 7.5, 1 MM MANGANOUS CHLORIDE, 5 % (W/V) POLY-ETHYLENEGLYCOL 6000, 0.1 M SODIUM CITRATE PH 5.
1		5		10 MM HEPES-NAOH PH 7.5, 1 MM MANGANOUS CHLORIDE, 5 % (W/V) POLY-ETHYLENEGLYCOL 6000, 0.1 M SODIUM CITRATE PH 5.