2XG8
Structural basis of gene regulation by protein PII: The crystal complex of PII and PipX from Synechococcus elongatus PCC 7942
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-11-10 |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 111.383, 61.585, 112.979 |
| Unit cell angles | 90.00, 126.44, 90.00 |
Refinement procedure
| Resolution | 50.000 - 3.200 |
| R-factor | 0.22923 |
| Rwork | 0.227 |
| R-free | 0.27247 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1v5h CHAIN J |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.259 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 90.900 | 3.370 |
| High resolution limit [Å] | 3.200 | 3.000 |
| Rmerge | 0.110 | 0.420 |
| Number of reflections | 10330 | |
| <I/σ(I)> | 7.1 | 1.8 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 3.9 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | PII-PIPX COMPLEX AT 5.5 MG/ML IN 20 MM HEPES PH 7.5, 1MM DTT, 0.4 M NACL, 5 MM MAGNESIUM CHLORIDE. PRECIPITANT SOLUTION: 15 % PEG 3.35K, 0.2 M SODIUM FORMATE. |
