2XG7
Crystal Structure of BST2-Tetherin Ectodomain expressed in HEK293T cells
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-04-30 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 28.860, 91.860, 146.960 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 77.890 - 3.450 |
| R-factor | 0.27038 |
| Rwork | 0.269 |
| R-free | 0.29795 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3MKX |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.379 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0074) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.950 | 3.640 |
| High resolution limit [Å] | 3.450 | 3.450 |
| Rmerge | 0.110 | 0.710 |
| Number of reflections | 5576 | |
| <I/σ(I)> | 8.9 | 2.5 |
| Completeness [%] | 99.2 | 99.4 |
| Redundancy | 6.8 | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 8% PEG8000, 0.1M TRIS-HCL PH 8.5 |
