2XG6
Molecular insights into clinically isolated OmpC mutants and their role in multi-drug resistance
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 210 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 142.820, 159.725, 164.601 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 53.190 - 3.470 |
| R-factor | 0.26278 |
| Rwork | 0.261 |
| R-free | 0.29306 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2xe1 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.948 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.6.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 54.000 | 3.700 |
| High resolution limit [Å] | 3.470 | 3.470 |
| Rmerge | 0.110 | 0.430 |
| Number of reflections | 40939 | |
| <I/σ(I)> | 7.1 | 1.8 |
| Completeness [%] | 84.6 | 73 |
| Redundancy | 4 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
