2XFE
vCBM60 in complex with galactobiose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 287 |
| Detector technology | CCD |
| Collection date | 2009-11-02 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 30.070, 49.540, 54.680 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.710 - 1.820 |
| R-factor | 0.14695 |
| Rwork | 0.145 |
| R-free | 0.19219 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | APO-VCBM60 |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.856 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0066) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.710 | 1.870 |
| High resolution limit [Å] | 1.820 | 1.820 |
| Rmerge | 0.060 | 0.140 |
| Number of reflections | 7772 | |
| <I/σ(I)> | 41.5 | 15.6 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 13.6 | 13.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 10MM GALACTOTRIOSE, 0.01M ZINC CHLORIDE, 0.1 M TRIS/HCL PH 8.0, 20% (W/V) PEG 6000 |
