2XF1

Crystal structure of Plasmodium falciparum actin depolymerization factor 1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX II BEAMLINE I911-2
Synchrotron site	MAX II
Beamline	I911-2
Temperature [K]	100
Detector technology	CCD
Collection date	2010-02-11
Detector	MARRESEARCH
Spacegroup name	P 32 2 1
Unit cell lengths	68.840, 68.840, 76.030
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	27.752 - 1.960
R-factor	0.1894
Rwork	0.187
R-free	0.22960
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB ENTRIES 1F7S 1AHQ AND 2I2Q
RMSD bond length	0.007
RMSD bond angle	1.003
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.050
High resolution limit [Å]	2.000	2.000
Rmerge	0.050	0.280
Number of reflections	14010
<I/σ(I)>	38.5	14.5
Completeness [%]	96.5	94.5
Redundancy	10.4	7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7.8	277	2.5 M AMMONUIM SULFATE, 0.1 M HEPES PH 7.8, 277 K