2XET

Conserved hydrophobic clusters on the surface of the Caf1A usher C-terminal domain are important for F1 antigen assembly

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	CCD
Collection date	2008-06-28
Detector	ADSC CCD
Spacegroup name	P 1 21 1
Unit cell lengths	39.669, 40.336, 44.862
Unit cell angles	90.00, 92.69, 90.00

Refinement procedure

Resolution	40.000 - 1.600
R-factor	0.1678
Rwork	0.167
R-free	0.20900
Structure solution method	SIRAS
Starting model (for MR)	NONE
RMSD bond length	0.012
RMSD bond angle	1.333
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHELX
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	1.640
High resolution limit [Å]	1.600	1.600
Rmerge	0.080	0.390
Number of reflections	17753
<I/σ(I)>	12.3	3.5
Completeness [%]	94.3	80.2
Redundancy	3.7	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		3		0.2M LISO4, 12-15% PEG8000, 4% PEG400, 0.1M NA-CITRATE PH2.8-3.0, PROTEIN 15 G/L, MIXED 1:1, MICROSEEDING
1		3		0.2M LISO4, 12-15% PEG8000, 4% PEG400, 0.1M NA-CITRATE PH2.8-3.0, PROTEIN 15 G/L, MIXED 1:1, MICROSEEDING