2XEA
4.6 ANGSTROM CRYO-EM RECONSTRUCTION OF TOBACCO MOSAIC VIRUS FROM IMAGES RECORDED AT 300 KEV ON A 4KX4K CCD CAMERA
EM specimen
| Vitrification | Yes |
| Instrument | HOMEMADE PLUNGER |
EM Data Collection
| Microscope | FEI TECNAI F30 |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2.3 |
| Detector | GATAN ULTRASCAN 4000 (4k x 4k) |
| Number of Images | 104 |
3D Reconstruction
| Method | HELICAL |
| Resolution (Å) | 4.6 |
| Software | Coot,UCSF Chimera,IMAGIC,SPIDER |
