2XDY
Crystal structure of the N. crassa QDE-2 AGO MID domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2010-02-15 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 80.100, 91.390, 42.930 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 31.288 - 2.200 |
R-factor | 0.1943 |
Rwork | 0.193 |
R-free | 0.22290 |
Structure solution method | SAD |
Starting model (for MR) | NONE |
RMSD bond length | 0.004 |
RMSD bond angle | 0.776 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | SHELX (C/D) |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 60.200 | 2.260 |
High resolution limit [Å] | 2.200 | 2.200 |
Number of reflections | 8285 | |
<I/σ(I)> | 18.4 | 2.94 |
Completeness [%] | 99.4 | 100 |
Redundancy | 3.6 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.2 | 200 MM AMMONIUM SULFATE 16% PEG4000, pH 7.2 |