2XDN

Transcription factor TtgR H67A mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I02
Synchrotron site	Diamond
Beamline	I02
Temperature [K]	100
Detector technology	CCD
Detector	ADSC CCD
Spacegroup name	P 1
Unit cell lengths	43.111, 43.174, 114.803
Unit cell angles	96.93, 99.26, 96.03

Refinement procedure

Resolution	41.739 - 2.200
R-factor	0.2219
Rwork	0.218
R-free	0.28790
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.180
Data reduction software	MOSFLM
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.700	2.320
High resolution limit [Å]	2.200	2.200
Rmerge	0.050	0.240
Number of reflections	37118
<I/σ(I)>	7.8	2.9
Completeness [%]	88.0	85.8
Redundancy	1.8	1.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1