2XDH

Non-cellulosomal cohesin from the hyperthermophilic archaeon Archaeoglobus fulgidus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	CCD
Collection date	2004-12-08
Detector	ADSC QUANTUM 210
Spacegroup name	P 43 3 2
Unit cell lengths	101.754, 101.754, 101.754
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.438 - 1.960
R-factor	0.1931
Rwork	0.189
R-free	0.22960
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB ENTRIES 1ANU 1aoh 1g1k 1qzn 1tyj
RMSD bond length	0.007
RMSD bond angle	1.093
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHENIX
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.030
High resolution limit [Å]	1.960	1.960
Rmerge	0.090	0.090
Number of reflections	13584
<I/σ(I)>	46.6	2.5
Completeness [%]	99.9	100
Redundancy	18.5	13.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1				0.1 M TRIS HYDROCHLORIDE PH 8.5, 2 M MONO-AMMONIUM DIHYDROGEN PHOSPHATE