2XDD
A processed non-coding RNA regulates a bacterial antiviral system
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-08-09 |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 182.850, 118.130, 41.900 |
| Unit cell angles | 90.00, 92.78, 90.00 |
Refinement procedure
| Resolution | 34.900 - 3.200 |
| R-factor | 0.194 |
| Rwork | 0.188 |
| R-free | 0.25600 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.010 |
| RMSD bond angle | 2.660 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((PHENIX.REFINE: 1.6_289)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 3.200 |
| High resolution limit [Å] | 3.200 | 3.100 |
| Rmerge | 0.110 | 0.380 |
| Number of reflections | 14694 | |
| <I/σ(I)> | 12.1 | 5 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 7.4 | 7.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 0.01M ZNSO4, 0.1M MES PH6.5, 25% PEG MME |
