2XDB
A processed non-coding RNA regulates a bacterial antiviral system
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-07-08 |
| Detector | ADSC CCD |
| Spacegroup name | I 41 3 2 |
| Unit cell lengths | 183.650, 183.650, 183.650 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.910 - 2.550 |
| R-factor | 0.178 |
| Rwork | 0.176 |
| R-free | 0.21200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2xdd |
| RMSD bond length | 0.009 |
| RMSD bond angle | 2.563 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 75.000 | 2.550 |
| High resolution limit [Å] | 2.550 | 2.500 |
| Rmerge | 0.150 | 0.780 |
| Number of reflections | 18604 | |
| <I/σ(I)> | 13.9 | 1.7 |
| Completeness [%] | 100.0 | 99.5 |
| Redundancy | 20 | 20.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 0.01M COCL2, 0.1M MES PH6.5, 1.8M (NH4)2SO4 |
