2XCX
Crystal structure of the apoform of the D52N variant of cytosolic 5'- nucleotidase II
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2008-01-30 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 92.120, 126.510, 130.080 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.680 - 2.300 |
| R-factor | 0.18476 |
| Rwork | 0.182 |
| R-free | 0.22901 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2jcm |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.548 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.400 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.070 | 0.600 |
| Number of reflections | 34076 | |
| <I/σ(I)> | 17.37 | 3.6 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 5.5 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.1 M BICINE PH9.0, 10% PEG6000 |
