2XCL
Nucleotide-bound Structures of Bacillus subtilis Glycinamide Ribonucleotide Synthetase
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-D |
| Synchrotron site | APS |
| Beamline | 14-BM-D |
| Temperature [K] | 100 |
| Collection date | 1998-07-11 |
| Detector | ADSC |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 56.890, 84.650, 85.180 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 26.980 - 2.100 |
| R-factor | 0.201 |
| Rwork | 0.201 |
| R-free | 0.26000 |
| Structure solution method | MAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.200 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.980 | 2.170 |
| High resolution limit [Å] | 2.090 | 2.090 |
| Rmerge | 0.080 | 0.280 |
| Number of reflections | 22361 | |
| <I/σ(I)> | 29.9 | 4.7 |
| Completeness [%] | 89.5 | 50.5 |
| Redundancy | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 30% PEG 4000, 0.1 M TRIS-HCL PH 8.5, 4.2 MM MGCL2, 1.8 MM AMP-PNP |
