2XCJ
Crystal structure of P2 C, the immunity repressor of temperate E. coli phage P2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-03-04 |
Detector | MARMOSAIC 225 mm CCD |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 91.103, 91.103, 80.754 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 35.000 - 1.800 |
R-factor | 0.15084 |
Rwork | 0.149 |
R-free | 0.17294 |
Structure solution method | SAD |
Starting model (for MR) | NONE |
RMSD bond length | 0.025 |
RMSD bond angle | 1.989 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHENIX (AUTOSOL) |
Refinement software | REFMAC (5.5.0044) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.000 | 1.900 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.070 | 0.670 |
Number of reflections | 32402 | |
<I/σ(I)> | 12.2 | 2 |
Completeness [%] | 100.0 | 100 |
Redundancy | 4.9 | 4.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 3.9M NA-FORMATE, 0.1M TRIS PH 7.5 |