2XCE
Structure of YncF in complex with dUpNHpp
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-03-07 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 99.116, 99.345, 99.252 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.850 |
| R-factor | 0.153 |
| Rwork | 0.151 |
| R-free | 0.18700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2xcd |
| RMSD bond length | 0.024 |
| RMSD bond angle | 2.007 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.080 | 1.950 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.010 | 0.940 |
| Number of reflections | 84193 | |
| <I/σ(I)> | 15.6 | 2.4 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 9.4 | 7.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 0.2M CACL2, 16% PEG 6K, 0.1M HEPES PH7 |
