2XCC
Crystal structure of PcrH from Pseudomonas aeruginosa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-12-07 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.950, 46.200, 65.570 |
| Unit cell angles | 90.00, 103.85, 90.00 |
Refinement procedure
| Resolution | 37.390 - 2.130 |
| R-factor | 0.26173 |
| Rwork | 0.258 |
| R-free | 0.29610 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vgx |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.713 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.260 |
| High resolution limit [Å] | 2.130 | 2.130 |
| Rmerge | 0.050 | 0.420 |
| Number of reflections | 89026 | |
| <I/σ(I)> | 30.3 | 3.88 |
| Completeness [%] | 89.0 | 65.8 |
| Redundancy | 6 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 0.1 M TRIS-HCL PH 8.5, 0.2 M MGCL2, AND 15% PEG4000 |
