2XC9
BINARY COMPLEX OF SULFOLOBUS SOLFATARICUS DPO4 DNA POLYMERASE AND 1, N2-ETHENOGUANINE MODIFIED DNA, MAGNESIUM FORM
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-BM |
Synchrotron site | APS |
Beamline | 22-BM |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2006-03-12 |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 93.722, 102.125, 53.004 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 26.910 - 2.200 |
R-factor | 0.221 |
Rwork | 0.221 |
R-free | 0.25000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2bq3 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.600 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 26.910 | 2.280 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.060 | 0.480 |
Number of reflections | 26245 | |
<I/σ(I)> | 36.3 | 6.2 |
Completeness [%] | 99.3 | 99.3 |
Redundancy | 12.6 | 10.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.5 | 12-20% POLYETHYLENE GLYCOL 3350, 0.2 M AMMONIUM ACETATE, 0.1 M MAGNESIUM ACETATE, AND 20 MM TRIS (PH 7.5) |